MDCAT Biology MCQ #1205

What is a major limitation of using bacteria as hosts for cloning large DNA fragments?

MDCAT Biology MCQ #1205

  1. Question 1

    Q1. What is a major limitation of using bacteria as hosts for cloning large DNA fragments?

    • A) Bacteria cannot take up large DNA molecules
    • B) Large DNA fragments are unstable in bacteria
    • C) Bacteria lack the machinery to replicate large DNA
    • D) Large DNA inserts often disrupt bacterial viability

    Answer: Large DNA fragments are unstable in bacteria

    Explanation: Large DNA fragments can be unstable in bacteria due to potential for recombination or deletion; Special vectors like BACs help mitigate this.