MDCAT Biology MCQ #3333

An enzyme has a Km of 0.1 mM and Vmax of 100 μmol/min. What is the most likely explanation if the enzyme's activity increases with higher substrate concentrations up to 1 mM?

MDCAT Biology MCQ #3333

  1. Question 1

    Q1. An enzyme has a Km of 0.1 mM and Vmax of 100 μmol/min. What is the most likely explanation if the enzyme's activity increases with higher substrate concentrations up to 1 mM?

    • A) The enzyme is saturated at 0.1 mM substrate
    • B) The enzyme is not saturated at 1 mM substrate
    • C) The enzyme has a very high affinity for the substrate
    • D) The enzyme is inhibited by the substrate at high concentrations

    Answer: The enzyme is not saturated at 1 mM substrate

    Explanation: Km is the substrate concentration at half Vmax, so the enzyme is not saturated at 1 mM