In the reaction of aldehydes with 2,4-DNPH, the pH must be carefully controlled. Why does the reaction fail in extremely acidic conditions?
Q1. In the reaction of aldehydes with 2,4-DNPH, the pH must be carefully controlled. Why does the reaction fail in extremely acidic conditions?
Answer: The amino group is protonated and loses nucleophilicity
Explanation: A pH of 3-4 is required for ammonia derivatives. Highly acidic conditions (pH 1) protonate the nitrogen, making it non-nucleophilic and inactive.